Research Interests
Our main interest is to understand the molecular mechanisms by which oncogenes transform a normal cell into a cancerous one. We focus our research on signaling pathways that are often deregulated in metabolic diseases and cancer, such as Ras/MAPK and PI3K/mTOR.
Using High-Throughput Proteomic Approaches to Identify Novel Effectors of the Ras/MAPK Pathway
Ras/MAPK signaling is activated by most growth factors and controls essential biological processes, including cell cycle progression, cell differentiation, survival, and motility. Activating mutations in components of this pathway are frequently found in human tumors. We have identified several novel proteins regulated by the Ras/MAPK pathway and are currently characterizing their roles in normal and cancer cells.
Assess the Biological Function of the RSK Family of Protein Kinases
The RSK family of protein kinases contains four human isoforms (RSK1-4). The RSK isoforms are directly activated by the Ras/MAPK pathway. Our group uses mouse genetics and molecular cell biology techniques to understand their roles in cancer. We have identified a large number of RSK substrates, which are currently being characterized as novel effectors of the Ras/MAPK pathway.
Characterize the Function and Regulation of the mTOR Signalling Pathway
The mammalian target of rapamycin (mTOR) is a conserved Ser/Thr kinase that regulates cell growth and organ size. Emerging evidence indicates that deregulation of the mTOR pathway occurs in many types of cancer. We have identified a number of new mTOR effectors and are currently characterizing their roles in cancer cell growth and proliferation.
Interplay Between Mechanical and Biological Mechanisms in Cell Cortex Assembly
The cell cortex is a network of actin, myosin, and associated proteins that underlie the plasma membrane and determine cell shape. Despite its importance, little is known about how the cortex assembles or its mechanical properties. This interdisciplinary project, in collaboration with Guillaume Charras and Ewa Paluch, aims to uncover the molecules involved in cortex assembly and explore their regulation.